Tsui et al. (1996) locirali su gen CRIP2 na hromosomsku poziciju 14q32, primjenom fluorescentne in situ hibridizacije (FISH).[9] Analizom somatskih hibridnih ćelija i FISH-metoda, Karim et al. (1996) mapirali su gen CRIP2 na poziciji 14q32.3.[10]
CRIP2 i usko povezani CRIP1 su proteini bogati cisteinom i sadrže po dva LIM-domena. Visoko su eksprimirani tokom kardiovaskularnog razvoja i djeluju na premošćavanje faktora serumskog odgovora i GATA i stimuliraju ciljne gene glatkih mišića.[11]
Skriningom niske strogosti ljudske biblioteke cDNKtankog crijeva cDNK bogatim cisteinom (CRIP1) cDNK, gorepomenuti Karim et al (1996) izolirali su cDNK koja kodira CRIP2, koju su nazvali ESP1/CRP2. cDNK predviđa protein od 208 aminokiselina koji sadrži dva LIM-domena. CRIP2 dijeli 93% identiteta aminokiselinske sekvence sa svojim homologom kod pacova, zvanim Esp1 ili Crp2. Northern blot analiza pokazala je široko rasprostranjenu tkivnu ekspresiju CRIP2 iRNK od 1,3 kb, s najvišim nivoom ekspresije u srcu. Također otkrivena je o druga kRNK u sjemenicima, od 1,7 kb.
Tsui et al. (1996) klonirali su cDNK ljudskog srca koja kodira CRIP2 i nazvali je CRP2. CRIP2 je 38%, odnosno 35% identičan proteinima CSRP1 i CSRP3.
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Tsui SK, Chan PP, Cheuk CW, Liew CC, Waye MM, Fung KP, Lee CY (Feb 1997). "A novel cDNA encoding for a LIM domain protein located at human chromosome 14q32 as a candidate for leukemic translocation". Biochem Mol Biol Int. 39 (4): 747–54. PMID8843343.
^Karim MA, Ohta K, Egashira M, Jinno Y, Niikawa N, Matsuda I, Indo Y (januar 1996). "Human ESP1/CRP2, a member of the LIM domain protein family: characterization of the cDNA and assignment of the gene locus to chromosome 14q32.3". Genomics. 31 (2): 167–76. doi:10.1006/geno.1996.0028. PMID8824798.
^Tsui, S. K. W., Chan, P. P. K., Cheuk, C. W., Liew, C. C., Waye, M. M. Y., Fung, K. P., Lee, C. Y. A novel cDNA encoding for a LIM domain protein located at human chromosome 14q32 as a candidate for leukemic translocation. Biochem. Molec. Biol. Int. 39: 747-754, 1996. PubMed: 8843343
^Karim, M. A., Ohta, K., Egashira, M., Jinno, Y., Niikawa, N., Matsuda, I., Indo, Y. Human ESP1/CRP2, a member of the LIM domain protein family: characterization of the cDNA and assignment of the gene locus to chromosome 14q32.3. Genomics 31: 167-176, 1996. PubMed: 8824798
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
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