GLYAT
Glicin-N-aciltransferaza, znana i kao GLYAT, je enzim koji je kod ljudi kodiran genom GLYAT.[5][6]
Aminokiselinska sekvenca
Dužina polipeptidnog lanca je 296 aminokiselina, a molekulska težina Da. 33 924[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MMLPLQGAQM | LQMLEKSLRK | SLPASLKVYG | TVFHINHGNP | FNLKAVVDKW | ||||
| PDFNTVVVCP | QEQDMTDDLD | HYTNTYQIYS | KDPQNCQEFL | GSPELINWKQ | ||||
| HLQIQSSQPS | LNEAIQNLAA | IKSFKVKQTQ | RILYMAAETA | KELTPFLLKS | ||||
| KILSPNGGKP | KAINQEMFKL | SSMDVTHAHL | VNKFWHFGGN | ERSQRFIERC | ||||
| IQTFPTCCLL | GPEGTPVCWD | LMDQTGEMRM | AGTLPEYRLH | GLVTYVIYSH | ||||
| AQKLGKLGFP | VYSHVDYSNE | AMQKMSYTLQ | HVPIPRSWNQ | WNCVPL |
Funkcija
Konjugati proteina glicin-N-aciltransferaze su glicin sa acil-CoA supstratima u mitohondrijama prvenstveno u jetri i bubrezima. Enzim glicin N-aciltransferaza uključen je u detoksikaciju širokog spektra ksenobiotika i endogenih metabolita. To uključuje benzoevu kiselinu, spoj koji se nalazi u voću i povrću i koristi se u medicini i prehrambenim proizvodima kao konzervans; salicilna kiselina, metabolit aspirina i nekoliko endogenih metabolita. Raznolikost se dokazuje širokim rasponom acilglicina koji se izlučuje urinom pacijenata s poremećajima metabolizma organskih kiselina. Još nije opisan nedostatak glicin N-aciltransferaze, ali je pokazano da postoje značajne međuindividualne varijacije u kapacitetu konjugacije glicina. Izoforma a-glicin N-aciltransferaze a je protein od 296 aminokiselina preveden iz prerađene varijante transkripta 1 iRNK. Kodiran je egzonima 2 do 6 transkripta iRNK.[5]
Molekulska težina
U literaturi se navodi da je molekulska težina ovog spoja približno 30 kDa.[6] ili približno 27 kDa.[8] Predviđena veličina je 33,9 KDa. Za goveđi enzim prijavljen je raspon veličina između približno 33 kDa i oko 36 KDa (Nandi, 1979., Vessey, 1992., van der Westhuizen, 2000.). Predviđena veličina goveđeg GLYAT-a, na osnovu njegove sekvence (pristupni broj nm: 177486), je 33,9 kDa. Ovo se dobro slaže s eksperimentalno utvrđenim veličinama.[8][9][10]
Reference
- ^ a b c GRCh38: Ensembl release 89: ENSG00000149124 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000063683 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b "Entrez Gene: GLYAT glycine-N-acyltransferase".
- ^ a b Mawal YR, Qureshi IA (decembar 1994). "Purification to homogeneity of mitochondrial acyl coa:glycine n-acyltransferase from human liver". Biochem. Biophys. Res. Commun. 205 (2): 1373–9. doi:10.1006/bbrc.1994.2817. PMID 7802672.
- ^ "UniProt, Q6IB77". Pristupljeno 22. 8. 2021.
- ^ a b van der Westhuizen FH, Pretorius PJ, Erasmus E (2000). "The utilization of alanine, glutamic acid, and serine as amino acid substrates for glycine N-acyltransferase". J. Biochem. Mol. Toxicol. 14 (2): 102–9. doi:10.1002/(SICI)1099-0461(2000)14:2<102::AID-JBT6>3.0.CO;2-H. PMID 10630424.
- ^ Nandi DL, Lucas SV, Webster LT (august 1979). "Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization". J. Biol. Chem. 254 (15): 7230–7. PMID 457678. Arhivirano s originala, 16. 11. 2007. Pristupljeno 22. 8. 2021.
- ^ Kelley M, Vessey DA (novembar 1992). "Structural comparison between the mitochondrial aralkyl-CoA and arylacetyl-CoA N-acyltransferases". Biochem. J. 288 (1): 315–7. doi:10.1042/bj2880315. PMC 1132116. PMID 1445276.
Dopunska literatura
- Webster LT, Siddiqui UA, Lucas SV, et al. (1976). "Identification of separate acyl- CoA:glycine and acyl-CoA:L-glutamine N-acyltransferase activities in mitochondrial fractions from liver of rhesus monkey and man". J. Biol. Chem. 251 (11): 3352–8. PMID 931988.
- Mawal YR, Qureshi IA (1994). "Purification to homogeneity of mitochondrial acyl coa:glycine n-acyltransferase from human liver". Biochem. Biophys. Res. Commun. 205 (2): 1373–9. doi:10.1006/bbrc.1994.2817. PMID 7802672.
- Mawal YR, Qureshi IA (1995). "An immunodetection method for the quantitation of human acyl CoA:glycine N-acyltransferase in biological samples". Biochem. Mol. Biol. Int. 34 (3): 595–601. PMID 7833837.
- Merkler DJ, Merkler KA, Stern W, Fleming FF (1996). "Fatty acid amide biosynthesis: a possible new role for peptidylglycine alpha-amidating enzyme and acyl-coenzyme A: glycine N-acyltransferase". Arch. Biochem. Biophys. 330 (2): 430–4. doi:10.1006/abbi.1996.0272. PMID 8660675.
- Mawal Y, Paradis K, Qureshi IA (1997). "Developmental profile of mitochondrial glycine N-acyltransferase in human liver". J. Pediatr. 130 (6): 1003–7. doi:10.1016/S0022-3476(97)70293-2. PMID 9202629.
- van der Westhuizen FH, Pretorius PJ, Erasmus E (2000). "The utilization of alanine, glutamic acid, and serine as amino acid substrates for glycine N-acyltransferase". J. Biochem. Mol. Toxicol. 14 (2): 102–9. doi:10.1002/(SICI)1099-0461(2000)14:2<102::AID-JBT6>3.0.CO;2-H. PMID 10630424.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- SCHACHTER D, TAGGART JV (2003). "Glycine N-acylase: purification and properties". J. Biol. Chem. 208 (1): 263–75. PMID 13174534.
- Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Vanjski linkovi
- GLYAT lokacija ljudskog genoma UCSC Genome Browser.
Ovaj članak ima dvojnika. Članak "Šablon:UCSC gen detalji" se također bavi ovom temom.
GLYAT human gene details in the UCSC Genome Browser.
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