PIWIL1
Piwi-liki protein 1 je protein koji je kod ljudi kodiran genom PIWIL1.[5][6][7]
Ovaj gen kodira člana potporodice PIWI argonaut proteina, evolucijski konzrrvirAnih proteina koji sadrže i PAZ i Piwi motive, sa važnom ulogom u samoobnavljanju matičnih ćelija , supresiji RNK i translacijskoj regulaciji u različitim organizmima.
Kodirani protein može imati ulogu unutrašnjeg regulatora sposobnosti samoobnavljanja zametne linije i hematopoetskih matičnih ćelija. Brojni geni identificirani su kao članovi porodice argonaut, u različitim neljudskim organizmima i smatra se da ti geni imaju važnu ulogu u razvoju i održavanju matičnih ćelija zametne linije. Ljudska porodica argonaut, sastoji se od osam članova. Proteini koji se proizvode od ovih članova porodice zadržavaju zajedničku arhitekturu sa PAZ motivom u sredini i Piwi motivom u C-terminalnoj regiji. Na osnovu poređenja sekvenci, osam članova porodice argonaut klasifikovano je u dvije potporodice: PIWI (PIWIL1/HIWI, PIWIL2/HILI, PIWIL3 i PIWIL4/HIWI2) i potporodicu eIF2C/AGO (EIF2C1/hAG hAGO2, EIF2C3/hAGO3 i EIF2C4/hAGO4). PCR analiza pomoću panela ljudskih cDNK s višestrukim tkivom pokazala je da su sva četiri člana PIWI potporodice eksprimirana uglavnom u sjemenicima, a sva četiri člana potporodice eIF2C/AGO u različitim tkivima odraslih. Eksperimenti imunoprecipitacije i vezivanja sa afinitetom koristeći ljudske HEK293 ćelije kotransfektirane cDNK za DICER sa oznakom FLAG, član porodice ribonukleaza III i članovi porodice argonaut označeni His-om, sugerirali su da su proteini iz članova obje potporodice povezani s DICER-om. Pretpostavlja se da bi barem neki članovi ljudske porodice argonauta mogli biti uključeni u razvoj i održavanje matičnih ćelijaa putem mehanizama za supresiju gena, posredovanih RNK, povezanih s DICER-om.[7]
Aminokiselinska sekvenca
Dužina polipeptidnog lanca je 861 aminokiselina, a molekulska težina 98.603 Da.[8].
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MTGRARARAR | GRARGQETAQ | LVGSTASQQP | GYIQPRPQPP | PAEGELFGRG | ||||
| RQRGTAGGTA | KSQGLQISAG | FQELSLAERG | GRRRDFHDLG | VNTRQNLDHV | ||||
| KESKTGSSGI | IVRLSTNHFR | LTSRPQWALY | QYHIDYNPLM | EARRLRSALL | ||||
| FQHEDLIGKC | HAFDGTILFL | PKRLQQKVTE | VFSKTRNGED | VRITITLTNE | ||||
| LPPTSPTCLQ | FYNIIFRRLL | KIMNLQQIGR | NYYNPNDPID | IPSHRLVIWP | ||||
| GFTTSILQYE | NSIMLCTDVS | HKVLRSETVL | DFMFNFYHQT | EEHKFQEQVS | ||||
| KELIGLVVLT | KYNNKTYRVD | DIDWDQNPKS | TFKKADGSEV | SFLEYYRKQY | ||||
| NQEITDLKQP | VLVSQPKRRR | GPGGTLPGPA | MLIPELCYLT | GLTDKMRNDF | ||||
| NVMKDLAVHT | RLTPEQRQRE | VGRLIDYIHK | NDNVQRELRD | WGLSFDSNLL | ||||
| SFSGRILQTE | KIHQGGKTFD | YNPQFADWSK | ETRGAPLISV | KPLDNWLLIY | ||||
| TRRNYEAANS | LIQNLFKVTP | AMGMQMRKAI | MIEVDDRTEA | YLRVLQQKVT | ||||
| ADTQIVVCLL | SSNRKDKYDA | IKKYLCTDCP | TPSQCVVART | LGKQQTVMAI | ||||
| ATKIALQMNC | KMGGELWRVD | IPLKLVMIVG | IDCYHDMTAG | RRSIAGFVAS | ||||
| INEGMTRWFS | RCIFQDRGQE | LVDGLKVCLQ | AALRAWNSCN | EYMPSRIIVY | ||||
| RDGVGDGQLK | TLVNYEVPQF | LDCLKSIGRG | YNPRLTVIVV | KKRVNTRFFA | ||||
| QSGGRLQNPL | PGTVIDVEVT | RPEWYDFFIV | SQAVRSGSVS | PTHYNVIYDN | ||||
| SGLKPDHIQR | LTYKLCHIYY | NWPGVIRVPA | PCQYAHKLAF | LVGQSIHREP | ||||
| NLSLSNRLYY | L |
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
Reference
- ^ a b c ENSG00000125207 GRCh38: Ensembl release 89: ENSG00000275051, ENSG00000125207 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000029423 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Cox DN, Chao A, Baker J, Chang L, Qiao D, Lin H (Jan 1999). "A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal". Genes Dev. 12 (23): 3715–27. doi:10.1101/gad.12.23.3715. PMC 317255. PMID 9851978.
- ^ Sasaki T, Shiohama A, Minoshima S, Shimizu N (Aug 2003). "Identification of eight members of the Argonaute family in the human genome small star, filled". Genomics. 82 (3): 323–30. doi:10.1016/S0888-7543(03)00129-0. PMID 12906857.
- ^ a b "Entrez Gene: PIWIL1 piwi-like 1 (Drosophila)".
- ^ "UniProt, Q96J94". Pristupljeno 12. 8. 2021.
Dopunska literatura
- Sharma AK, Nelson MC, Brandt JE, et al. (2001). "Human CD34(+) stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi". Blood. 97 (2): 426–34. doi:10.1182/blood.V97.2.426. PMID 11154219.
- Qiao D, Zeeman AM, Deng W, et al. (2002). "Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas". Oncogene. 21 (25): 3988–99. doi:10.1038/sj.onc.1205505. PMID 12037681.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Tahbaz N, Kolb FA, Zhang H, et al. (2004). "Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer". EMBO Rep. 5 (2): 189–94. doi:10.1038/sj.embor.7400070. PMC 1298981. PMID 14749716.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Liu X, Sun Y, Guo J, et al. (2006). "Expression of hiwi gene in human gastric cancer was associated with proliferation of cancer cells". Int. J. Cancer. 118 (8): 1922–9. doi:10.1002/ijc.21575. PMID 16287078. S2CID 8616640.
- Taubert H, Greither T, Kaushal D, et al. (2007). "Expression of the stem cell self-renewal gene Hiwi and risk of tumour-related death in patients with soft-tissue sarcoma". Oncogene. 26 (7): 1098–100. doi:10.1038/sj.onc.1209880. PMID 16953229.
- Sugimoto K, Kage H, Aki N, et al. (2007). "The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus". Biochem. Biophys. Res. Commun. 359 (3): 497–502. doi:10.1016/j.bbrc.2007.05.136. PMID 17544373.
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