Kada su mali jedarni ribonukleoproteini U1 i U2 (snRNP) prečišćeni postupkom koji čuva njihovu imunoprecipitabilnost autoimunskim antitijelima, podvrgnuti opsežnoj probavi mikrokoknom nukleosomom, otkriveno je da njihov obrazac razgradnje oštro ovisi o koncentraciji magnezija, što ukazuje na to da podliježu dubokim strukturnim promjenama. Pri niskom Mg2+ (manje ili jednako 5 mM), obje čestice pokazuju samo jezgrenu otpornu strukturu za koju je prethodno utvrđeno da je zajednička za sve osim za U6 snRNK .
Pri visokim Mg2+ (većim ili jednakim 7 mM), U1 i U2 snRNP ponašaju se međusobno različito. U U1 snRNP, većina sekvenci U1 snRNK je zaštićena, osim 10 5'-terminalnih nukleotida, za koje se pretpostavlja da su uključeni u preradu i kratke sekvence između nukleotida 102 i 108. Druga regija koja se proteže od nukleotida 60 do 79 je samo slabo zaštićena. Pokazalo se da je ova strukturna modifikacija reverzibilna. U U2 snRNP, sekvenca U2 snRNK ostaje izložena u svom 5' dijelu do nukleotida 92, a 3'-terminalna ukosnica smještena izvan strukture jezgra postaje zaštićena.[3]
Reference
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^I Reveillaud, M N Lelay-Taha, J Sri-Widada, C Brunel, P Jeanteur (1984):
Mg2+ induces a sharp and reversible transition in U1 and U2 small nuclear ribonucleoprotein configurations. Mol Cell Biol, 4(9):1890-1899; pmid: 6238232; pmcid: pmc368999; doi: 10.1128/mcb.
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