YAF2
YY1-pridruženi faktor 2 je protein koji je kod ljudi kodiran genom YAF2.[5][6] Gen YAF2 mapiran je na hromosomu 12q12, na osnovu poravnavanja sekvence YAF2 (GenBank BC037777) sa genomskom sekvencom (GRCh38).
Protein kodiran ovim genom stupa u interakciju s YY1, proteinom cinkovog prsta, koji je uključen u negativnu regulaciju gena ograničenih na mišiće. Sam ovaj genski proizvod sadrži jedan N-terminalni C2-X10-C2 cinkov prst, i za razliku od YY1, nsdreguliran je tokom miogene diferencijacije. Također olakšava proteolitsko cijepanje YY1 pomoću proteaze aktivirane kalcijem, m-kalpaina, sugerirajući mehanizam pomoću kojeg ovaj protein antagonizira negativaom učinku YY1.[6]
Aminokiselinska sekvenca
Dužina polipeptidnog lanca je 180 aminokiselina, a molekulska težina 19.901 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MGDKKSPTRP | KRQPKPSSDE | GYWDCSVCTF | RNSAEAFKCM | MCDVRKGTST | ||||
| RKPRPVSQLV | AQQVTQQFVP | PTQSKKEKKD | KVEKEKSEKE | TTSKKNSHKK | ||||
| TRPRLKNVDR | SSAQHLEVTV | GDLTVIITDF | KEKTKSPPAS | SAASADQHSQ | ||||
| SGSSSDNTER | GMSRSSSPRG | EASSLNGESH |
Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
Kloniranje i ekspresija
Koristeći YY1 kao sondu u 2-hibridnom kvascu, Kalenik et al. (1997) klonirali YAF2 iz biblioteke cDNK mišića. Izvedeni protein od 180 aminokiselina ima izračunatu molekulsku masu od oko 20 kD. Sadrži N-terminalni motiv cinkovog prsta tipa C2-X10-C2, gdje je X bilo koja aminokiselina, i navodni signal jedarne lokalizacije. YAF2 dijeli homologiju ograničene sekvence s nekoliko nukleoporina i RAN-vezujućeg proteina-2 (RANBP2).[8]
Prema Northern blot analizi, Sawa et al. (2002) otkrili su ekspresiju YAF2 u mnogim tkivima, s najvećom ekspresijom u srcu i skeletnim mišićima. Uočili su homologiju između YAF2 i RYBP, posebno unutar njihovih N-terminalnih domena.[9]
Funkcija gena
Kalenik et al. (1997.) utvrdili su da dva prva cinkova prsta YY1 vežu YAF2. Razina YAF2 iRNK povećala se tokom in vitro diferencijacije ćelija skeleta i srčanog mišića pacova, za razliku od smanjene regulacije YY1. Prekomjerna ekspresija YAF2 u ćelijama mišjih mioblasta stimulirala je aktivnost miogenog promotora. Kotransfekcija i ekspresija YY1 u ovim ćelijama preokrenuli su stimulacijski učinak YAF2. YAF2 je također povećao proteolitsko cijepanje YY1 kalcija – aktiviranom proteazom m-kalpaina.
Kotransfekcijom i ekspresijom YAF2, GABPA i GABPB u ćelijama insekata, Sawa et al. (2002) utvrdili su da je YAF2 transkripcijski koaktivator za GABP.
Reference
- ^ a b c GRCh38: Ensembl release 89: ENSG00000015153 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000022634 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Kalenik JL, Chen D, Bradley ME, Chen SJ, Lee TC (Mar 1997). "Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1". Nucleic Acids Res. 25 (4): 843–9. doi:10.1093/nar/25.4.843. PMC 146511. PMID 9016636.
- ^ a b "Entrez Gene: YAF2 YY1 associated factor 2".
- ^ "UniProt, Q8IY57". Pristupljeno 8. 8. 2021.
- ^ Kalenik, J. L., Chen, D., Bradley, M. E., Chen, S.-J., Lee, T.-C. Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1. Nucleic Acids Res. 25: 843-849, 1997. PubMed: 9016636
- ^ Sawa, C., Yoshikawa, T., Matsuda-Suzuki, F., Delehouzee, S., Goto, M., Watanabe, H., Sawada, J., Kataoka, K., Handa, H. YEAF1/RYBP and YAF-2 are functionally distinct members of a cofactor family for the YY1 and E4TF1/hGABP transcription factors. J. Biol. Chem. 277: 22484-22490, 2002. PubMed: 11953439
Dopunska literatura
- Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Mädge B, Geisen C, Möröy T, Schwab M (2003). "Yaf2 inhibits Myc biological function". Cancer Lett. 193 (2): 171–6. doi:10.1016/S0304-3835(02)00696-1. PMID 12706874.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ogawa H, Ishiguro K, Gaubatz S, et al. (2002). "A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells". Science. 296 (5570): 1132–6. doi:10.1126/science.1069861. PMID 12004135. S2CID 34863978.
- Sawa C, Yoshikawa T, Matsuda-Suzuki F, et al. (2002). "YEAF1/RYBP and YAF-2 are functionally distinct members of a cofactor family for the YY1 and E4TF1/hGABP transcription factors". J. Biol. Chem. 277 (25): 22484–90. doi:10.1074/jbc.M203060200. PMID 11953439.
- Bannasch D, Mädge B, Schwab M (2001). "Functional interaction of Yaf2 with the central region of MycN". Oncogene. 20 (41): 5913–9. doi:10.1038/sj.onc.1204747. PMID 11593398.
Vanjski linkovi
- YAF2 lokacija ljudskog genoma UCSC Genome Browser.
Ovaj članak ima dvojnika. Članak "Šablon:UCSC gen detalji" se također bavi ovom temom.
YAF2 human gene details in the UCSC Genome Browser.
Content Disclaimer
Informasi ini disarikan dari Wikipedia dan disajikan kembali untuk tujuan edukasi. Konten tersedia di bawah lisensi CC BY-SA 3.0. Kami tidak bertanggung jawab atas ketidakakuratan data yang bersumber dari kontribusi publik tersebut.
- The information displayed on this website is sourced in part or in whole from Wikipedia and has been adapted for the purpose of restating it. We strive to provide accurate and relevant information, however:
- There is no guarantee of absolute accuracy. Wikipedia is an open, collaborative project that can be edited by anyone, so information is subject to change.
- It is not intended to constitute professional advice. The content displayed is for informational and educational purposes only. For important decisions (e.g., medical, legal, or financial), please consult a professional.
- Content copyright. Wikipedia is licensed under the Creative Commons Attribution-ShareAlike License (CC BY-SA). This means that content may be reused with appropriate attribution and shared under a similar license.
- Responsible use. Any risk arising from the use of information from this website is entirely the responsibility of the user.
